The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. These applications may make use of…FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. 1 sec. Customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 2. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 303 unable to cal dark current. Products : General, Microplate Readers. Select the Driver tab. 9. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). See More Microplate Reader / Microtiter Plate Reader. QuickSync. The plate is set up for reading and images are acquired according to specified parameters. This application note compares the performance of the Molecular Devices EMax ® Plus Microplate. READ ANALYZE IMPORTa single plate read using any standard cuvette, or 96- or 384-well microplate. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader ( Molecular Devices, San Jose, CA, USA) at 30 °C. 5: Schematic of the back panel of a reader. The automated ELISA workflow has two positive outcomes. It also allows for the elimination of standard curves. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. , June 1, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of FLIPRTETRA the first system that enables researchers to significantly increase throughput of cell-based assays by measuring 1536 kinetic measurements simultaneously. Read More. Next generation SpectraMax® absorbance microplate readers. 1700 +1 800. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Plate Reader. Cell line development teams will realize savings on time and resources,Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Read one sample or up to 384 in a. Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial Rack Bottom Label 2D Reader) Microscan: MS-3 Laser Bar Code Reader: Thermo Fisher Scientific: VisionMate 96 (Vial Rack Bottom Label 2D Reader). It features a modular design that allows users to add or change detection cartridges according to their needs. Catalog Number i3x. VMax . Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. VMax® Microplate Readers. 40266 Email: dru[email protected] iD5 reader’s filter system. 1. Plate pre-read measurements were made in a Costar UV plate. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Never order or replace filters to optimize an assay again!SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Molecular Devices' microplate readers. ELISA Plate Readers / Microplate Readers. The computer controlling the plate reader runs Windows XP. Lower variance: Normalize your plate reader assay data to the number of cells in each well. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Create custom fluorescence assays without having to purchase expensive filter sets. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Under "Category", select "Plate Type". 0. You can also contact Technical Support by email. begin reading microplates with seven mouse clicks. Upgradeable modules are also available. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. STAKMAX. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. Instrument settings can be saved as a protocol file. Microplate readers and controlling software enable walk away data analysis. * Note: Programming the washer with a final aspirate height of 4. 1 second up to 30 seconds. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode SpectraMax® Microplate Reader User Manual Download Page. Cellular Imaging Systems. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Gregory et al. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. To purchase a newer software version,. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. NORTH AMERICA. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. Catalog Number: (89212-396) Supplier: Molecular Devices. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. We would like to show you a description here but the site won’t allow us. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 1. The guide also covers the software features, the specifications, and the accessories of the systems. Absorbance, fluorescence, and luminescence detection with upgradeable high-performance capabilities. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Shop now. Microplate Reader, Multimode. The system features 96, 384, and 1536 simultaneous liquid transfer, multi-wavelength kinetic reading, fluorescence and luminescence detection, agile internal plate handling, and elevated instrument0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. Also refer to the Multimode Reader Comparison Chart Features. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersMolecular Devices technical support. Page 1 of 8 . Select Update Driver. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. The content of this site is published by the site. 1. The opaque walls prevent stray light from being detected from neighbouring. Page 1 of 8 . Instrument settings can be saved as a protocol file. Three low volume 24-well bottom slides. With The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. A control well was set up to verify the accuracy of the standard curve. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). These systems are hybrids that allow you to choose between. 6") Weight. GxP regulated industry assessments of microplate readers. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. Published Dec 16, 2020 | Updated May 19, 2023. The most published microplate reader control and data analysis software. The Versamax Microplate Reader is built around a grating monochromator. Two different agonists, acetylcholine and carbachol, were tested. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. 1. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 5. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Experimental details and customization of the group table. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. , call 1-800-635-5577. Contact our support team with your questions regarding Molecular Devices products and applications. Some familiarity with Windows should be sufficient to use it. sensitivity or performance. Table of Contents. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. Since cells are nearly confluent, the protocol. Absorbance Plate Reader Comparison Chart. The instrument will read theThe FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Jun 01, 2004. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Details. SoftMax Pro 7. The FlexStation® Scanning Fluorometer (Molecular Devices; Fig. We would like to show you a description here but the site won’t allow us. 100 μL of blank or endotoxin standard (in. The adapter is required for optimum performance with standard 96-well and 384-. Lab automation for high-throughput plate-based assays. Cellular Imaging Systems. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Temperature Control 4°C above ambient to 45°C. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesPlate reader instructions Computer. Please click on the links below to view the Microplate Reader Comparison Tables. However, when a specific probe is not available, or it causes off-target effects on the. Recombinant protein expression Nucleocapsid. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. 0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Description Figure 1. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 0. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Used. The drawing shows a standard microplate of 15 mm height covered with a lid. 9. 00. 1 up to 3600 sec. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. Cells in each. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. EUROPE. 2. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. . Select the Driver tab and click Update Driver. 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. This can increase throughput and efficiency, thus saving time and space in the lab. #6590-0118) Filter 665/10 (Molecular Devices cat. Gemini EM is a microplate spectrofluorometer from Molecular Devices. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. 0) OD (0. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. 40-plate magazine (pair) 0200-6017. 1. Unplug the power cable from the back of the instrument. 1 sec. SoftMax Pro 7. Price Get Quote. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. Get a front row view of your cells. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Some familiarity with Windows should be sufficient to use it. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Step 2: Launch SoftMax Pro Software. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Figure 1. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. . EMax. 4 °C above ambient to 45 °C. . Data analysis. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. 340 PC384. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Import plate-based data from any vendor SoftMax Pro enables you to import data from acquisition sources other than a Molecular Devices microplate reader. M2. Versa. Item SpectraMax i3x Multi-Mode Microplate Reader. in steps of 0. . Fluorescence Microplate Reader Comparison Chart. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Turn the reader on and secure the USB cable. 1. D. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Molecular Devices SpectraMax Gemini XS microplate reader with laptop and software (Pre-owned) $5,500. FLIPR Calcium 4 Assay Kit. Eight filter options provide simplified assay setup. We would like to show you a description here but the site won’t allow us. SpectraDrop High-Throughput Screening (HTS) Kit. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. 1. 0) OD (500 to 1100) RFU (5. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. For more sample throughput, both readers can be easily. Incubate at RT. Reader Documentation Version No. integration time per well, depending on firmware version. Register to download your free eBook today. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 1 - 16 of 69. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. 9000-0756. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Select Update Driver. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Read height should be optimized forVmax® plate reader specifications. The advantage of using labeled probes is detection specificity. +44-118-944-8000. 3860 N First Street San Jose,CA 95134 USA +1 408. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cells in each image are identified by SoftMax Pro Software and cell-by-cellOne plate carrier adapter is provided with the instrument. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Search. Ratios for each sample were calculated as Read B/Read A. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. or 0. Uncontrolled Copy for Training and Reference Purposes Only . The plate reader optics are tunable for absorbance. Molecular Devices, LLC. 2. (Figure 1). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A)Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 2. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 13. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Shortside barcode reader: 0310-5102. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Plate Formats 6 to 1536 wells. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. SoftMax® Pro 7. Maintaining data integrity is a. Molecular Devices. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. For nearly 30 years, Molecular Devices has provided scientists with . Learn how Molecular Device's VersaMax Microplate Reader can be combined with the Pyrochrome Assay from Associates of Cape Cod, Inc. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Here, we describe a novel GCaMP6s-CAAX-based calcium assay utilizing a high-throughput fluorometric imaging plate reader (Molecular Devices, Sunnyvale, CA) format that can identify both activators and inhibitors of T-type Ca 2+ channels. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. , target CV per well = 1. 312 gain calibration failed. W×D×H. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. Instrument settings can be saved as a protocol file. Data collection speed is sufficient for running fast cell-based kinetic assays. Scanning options are endpoint, kinetic, spectral. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Multi-mode Plate Reader. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. 18 kg. The Back Panel Figure 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 190. Supplier: Molecular Devices. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Flexibility to read a variety of plate formats. VIS absorbance detection. 3860 N First Street San Jose, CA 95134. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). If it looks fine, then proceed. Plate reader instructions Computer. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. DOCUMENTATION. Introduction. Use a Phillips screwdriver to remove the screws, and then remove the plate from the instrument. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. View the Molecular Devices website. The Back Panel Figure 1. 1. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. gene expression with a user-friendly reader. For more sample throughput, the system can be easily integrated into a full roboticOnly Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Tests every subsystem of the reader or . VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 9. Automated microplate readers reduce manual manipulation and ensure consistent operation. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Clone Screening.